The molecular ecologist's guide to expressed sequence tags
نویسندگان
چکیده
منابع مشابه
The molecular ecologist's guide to expressed sequence tags.
Genomics and bioinformatics have great potential to help address numerous topics in ecology and evolution. Expressed sequence tags (ESTs) can bridge genomics and molecular ecology because they can provide a means of accessing the gene space of almost any organism. We review how ESTs have been used in molecular ecology research in the last several years by providing sequence data for the design ...
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Now a days, genome analysis has employed a rapid analysis tool known as expressed sequence tags. In 1983, SD Putney for the first time demonstrated the use of cDNA in identification of genome. The term expressed sequence tags (ESTs) was coined by Anthony Kerlavage at the Institute for Genomic Research. In 1991, Mark Adams used EST in relation to gene discovery and Human Genome project. cDNA or ...
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Thirty years ago, marine microbes were described by crude morphology and the ability to grow on different carbon sources. Our understanding of their ecological role in aquatic environments was murky at best. Since then, the development of new molecular methods facilitated by DNA sequencing resulted in a revolution in the field of microbial molecular ecology and evolution. Plummeting sequencing ...
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Expressed sequence tags (ESTs) present a special set of problems for bioinformatic analysis. They are partial and error-prone, and large datasets can have significant internal redundancy. To facilitate analysis of small EST datasets from in-house projects, we present an integrated "pipeline" of tools that take EST data from sequence trace to database submission. These tools also can be used to ...
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The domestic apple (Malus domestica; also known as Malus pumila Mill.) has become a model fruit crop in which to study commercial traits such as disease and pest resistance, grafting, and flavor and health compound biosynthesis. To speed the discovery of genes involved in these traits, develop markers to map genes, and breed new cultivars, we have produced a substantial expressed sequence tag c...
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ژورنال
عنوان ژورنال: Molecular Ecology
سال: 2006
ISSN: 0962-1083,1365-294X
DOI: 10.1111/j.1365-294x.2006.03195.x